Journal: Cells

Article Title: Therapeutic Potential of Stearoyl-CoA Desaturase1 (SCD1) in Modulating the Effects of Fatty Acids on Osteoporosis

doi: 10.3390/cells13211781

Figure Lengend Snippet: Schematic diagram illustrating the research concept. In osteoporosis models, fatty acids such as SFA and MUFA are secreted from expanding MAT. This study suggests that SCD1 can promote bone regeneration by converting SFA, which is known to have a negative effect on osteoblast differentiation and MSC proliferation, into MUFA, which can have a positive effect on bone metabolism. This highlights the therapeutic potential of MSCs through SCD1 overexpression in bone regeneration in osteoporosis models.

Article Snippet: The plasmid containing the cDNA of human SCD1 (NM_005063) (pCMV6-XL5-SCD1) was purchased from Origene.

Techniques: Over Expression

Journal: Cells

Article Title: Therapeutic Potential of Stearoyl-CoA Desaturase1 (SCD1) in Modulating the Effects of Fatty Acids on Osteoporosis

doi: 10.3390/cells13211781

Figure Lengend Snippet: Effect of SCD1 overexpression on osteogenic differentiation reduced by palmitic acid (PA). ( A ) Production of adenovirus-based SCD1 vector. ( B ) Confirmation of cell viability of BM-MSCs according to virus MOI. ( C ) Verification of MOI-dependent overexpression of Ad-SCD1. ( D ) Confirmation of normal virus infection and GFP expression in BM-MSCs. ( E ) Measurement of cell viability with the virus. ( F – I ) Confirmation of the effect of Ad-SCD1 on osteogenic differentiation of PA-treated BM-MSCs through comparison of ALP and ARS staining, ALP activity, and calcium deposition. (*** p < 0.001).

Article Snippet: The plasmid containing the cDNA of human SCD1 (NM_005063) (pCMV6-XL5-SCD1) was purchased from Origene.

Techniques: Over Expression, Plasmid Preparation, Virus, Infection, Expressing, Comparison, Staining, Activity Assay

Journal: Cells

Article Title: Therapeutic Potential of Stearoyl-CoA Desaturase1 (SCD1) in Modulating the Effects of Fatty Acids on Osteoporosis

doi: 10.3390/cells13211781

Figure Lengend Snippet: In vivo experiments to verify the effect of SCD1 on bone defect recovery in an osteoporosis model. ( A ) Schematic diagram of the animal modeling experiment. ( B ) X-ray scan images of the left distal femur at 1, 3, 5, and 7 weeks after cell transplantation into the defect. ( C ) BMD measurements by DEXA imaging at 1, 3, 5, and 7 weeks (* p < 0.05, *** p < 0.001).

Article Snippet: The plasmid containing the cDNA of human SCD1 (NM_005063) (pCMV6-XL5-SCD1) was purchased from Origene.

Techniques: In Vivo, Transplantation Assay, Imaging